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Hypoxyprobe公司基于缺氧探針抗體技術(shù),在此技術(shù)上開發(fā)了系列缺氧探針標(biāo)記試劑盒,為組織疾病的診斷和治療提供有力研究工具。
檢測(cè)原理
低氧標(biāo)志物測(cè)定是利用還原硝基有選擇性結(jié)合乏氧細(xì)胞的能力而形成的一種從細(xì)胞水平測(cè)定腫瘤乏氧的技術(shù),其原理是:腫瘤乏氧細(xì)胞還原能力強(qiáng),當(dāng)具有電子親合力的硝基咪唑主動(dòng)擴(kuò)散透過細(xì)胞脂膜,在細(xì)胞內(nèi)硝基還原酶作用下,硝基被還原,還原產(chǎn)物與大分子物質(zhì)不可逆結(jié)合,滯留在組織內(nèi)。而正常含氧細(xì)胞親電子硝基咪唑還原產(chǎn)物立即被氧化,從而用硝基咪唑類化合物在腫瘤內(nèi)的代謝程度來反映腫瘤的乏氧
Pimonidazole是新型的乏氧細(xì)胞標(biāo)記物,HPI公司擁有硝基咪唑類化合物特異性抗體,該抗體為公司技術(shù),可用于免疫組織化學(xué)、酶聯(lián)免疫吸附試驗(yàn)和流式細(xì)胞分析檢測(cè)。
Pimonidazole 應(yīng)用
特異性的低氧探(Hypoxyprobe-1)zui初用于反映腫瘤細(xì)胞內(nèi)的低氧梯度,正常細(xì)胞無表達(dá)或低表達(dá)。而在低氧的腫瘤細(xì)胞內(nèi)可見高表達(dá),并且隨低氧的程度越重而顏色越深。由于其很好的化學(xué)穩(wěn)定性、水溶性,同時(shí)小分子的探針可以自由穿過血腦屏障而到達(dá)腦組織細(xì)胞及腦腫瘤細(xì)胞。在實(shí)驗(yàn)中應(yīng)用低氧探針可以形象直觀的通過免疫組化來分析腦挫傷周圍組織細(xì)胞低氧的范圍和程度,同時(shí)對(duì)實(shí)驗(yàn)設(shè)備及器材要求較低,為無創(chuàng)性實(shí)驗(yàn)。
中文名稱 | 貨號(hào) | 產(chǎn)品描述 | 包裝 |
派諾硝唑探針&抗派諾硝唑探針小鼠單抗(不帶標(biāo)記) | HP1-100Kit | 100 mg pimonidazole HCl plus 1.0 ml of 4.3.11.3 mouse Mab | 1 kit |
派諾硝唑探針&抗派諾硝唑探針小鼠單抗(不帶標(biāo)記) | HP1-200Kit | 200 mg pimonidazole HCl plus 1.0 ml of 4.3.11.3 mouse Mab | 1 kit |
派諾硝唑探針&抗派諾硝唑探針小鼠單抗(不帶標(biāo)記) | HP1-1000Kit | 1000 mg pimonidazole HCl plus 2 x 1.0 ml of 4.3.11.3 mouse Mab | 1 kit |
派諾硝唑探針&FTIC-抗派諾硝唑探針小鼠單抗&HRP- 兔抗FITC抗體 | HP2-100Kit | 100 mg pimonidazole HCl plus 1 unit of 4.3.11.3 mouse FITC-MAb and 1 unit of anti-FITC HRP | 1 kit |
派諾硝唑探針&FTIC-抗派諾硝唑探針小鼠單抗&HRP- 兔抗FITC抗體 | HP2-200Kit | 200 mg pimonidazole HCl plus 1 unit of 4.3.11.3 mouse FITC-MAb and 1 unit of anti-FITC HRP | 1 kit |
派諾硝唑探針&FTIC-抗派諾硝唑探針小鼠單抗&HRP- 兔抗FITC抗體 | HP2-1000Kit | 1000 mg pimonidazole HCl plus 2 units of 4.3.11.3 mouse FITC-MAb and 2 units of anti-FITC HRP | 1 kit |
派諾硝唑探針&抗派諾硝唑探針兔多抗(不帶標(biāo)記) | HP3-100Kit | 100 mg pimonidazole HCl plus 1 unit of 2627 rabbit antisera | 1 kit |
派諾硝唑探針&抗派諾硝唑探針兔多抗(不帶標(biāo)記) | HP3-200Kit | 200 mg pimonidazole HCl plus 1 unit of 2627 rabbit antisera | 1 kit |
派諾硝唑探針&抗派諾硝唑探針兔多抗(不帶標(biāo)記) | HP3-1000Kit | 1000 mg pimonidazole HCl plus 2 units of 2627 rabbit antisera | 1 kit |
CCI-103 探針&抗CCI-103F兔多抗 | HP4-100Kit | 100 mg CCI-103F (F6) plus 1 unit of anti-F6 rabbit antisera | 1 kit |
CCI-103 探針&抗CCI-103F兔多抗 | HP4-200Kit | 200 mg CCI-103F (F6) plus 1 unit of anti-F6 rabbit antisera | 1 kit |
CCI-103 探針&抗CCI-103F兔多抗 | HP4-1000Kit | 1000 mg CCI-103F (F6) plus 2 units of anti-F6 rabbit antisera | 1 kit |
派諾硝唑探針&CCI-103探針&抗派諾硝唑探針&CCI-103兔多抗 | HP5-100Kit | 100 mg each of pimonidazole HCl and CCI-103F (F6) plus 1 unit of each rabbit antisera | 1 kit |
派諾硝唑探針&CCI-103探針&抗派諾硝唑探針&CCI-104兔多抗 | HP5-200Kit | 200 mg each of pimonidazole HCl and CCI-103F (F6) plus 1 unit of each rabbit antisera | 1 kit |
派諾硝唑探針&CCI-103探針&抗派諾硝唑探針&CCI-105兔多抗 | HP5-1000Kit | 1000 mg each of pimonidazole HCl and CCI-103F plus 2 units of each rabbit antisera | 1 kit |
派諾硝唑探針&FITC-抗派諾硝唑探針小鼠單抗 | HP6-100Kit | 100 mg pimonidazole HCl plus 1 unit of 4.3.11.3 mouse FITC-Mab | 1 kit |
派諾硝唑探針&FITC-抗派諾硝唑探針小鼠單抗 | HP6-200Kit | 200 mg pimonidazole HCl plus 1 unit of 4.3.11.3 mouse FITC-Mab | 1 kit |
派諾硝唑探針&FITC-抗派諾硝唑探針小鼠單抗 | HP6-1000Kit | 1000 mg pimonidazole HCl plus 2 units of 4.3.11.3 mouse FITC-Mab | 1 kit |
派諾硝唑探針&Dylight™549標(biāo)記-抗派諾硝唑探針小鼠單抗 | HP7-100Kit | 100 mg pimonidazole HCl plus 1 unit of 4.3.11.3 mouse Dylight?49-Mab | 1 kit |
派諾硝唑探針&Dylight™549標(biāo)記-抗派諾硝唑探針小鼠單抗 | HP7-200Kit | 200 mg pimonidazole HCl plus 1 unit of 4.3.11.3 mouse Dylight?49-Mab | 1 kit |
派諾硝唑探針&Dylight™549標(biāo)記-抗派諾硝唑探針小鼠單抗 | HP7-1000Kit | 1000 mg pimonidazole HCl plus 2 units of 4.3.11.3 mouse Dylight?49-Mab | 1 kit |
派諾硝唑探針&別藻藍(lán)素標(biāo)記-抗派諾硝唑探針小鼠單抗 | HP8-100Kit | 100 mg pimonidazole HCl plus 1 unit of 4.3.11.3 mouse Allophycocyanin-Mab | 1 kit |
派諾硝唑探針&別藻藍(lán)素標(biāo)記-抗派諾硝唑探針小鼠單抗 | HP8-200Kit | 200 mg pimonidazole HCl plus 1 unit of 4.3.11.3 mouse Allophycocyanin-Mab | 1 kit |
派諾硝唑探針&別藻藍(lán)素標(biāo)記-抗派諾硝唑探針小鼠單抗 | HP8-1000Kit | 1000 mg pimonidazole HCl plus 2 units of 4.3.11.3 mouse Allophycocyanin-Mab | 1 kit |
派諾硝唑探針&生物素標(biāo)記-抗派諾硝唑探針小鼠單抗 | HP10-100kit | Hypoxyprobe-Biotin Kit (100 mg pimonidazole HCl plus 2 units of 200ul of Mouse Biotin fluorophore (HP-Biotin)) | 1 kit |
派諾硝唑探針&生物素標(biāo)記-抗派諾硝唑探針小鼠單抗 | HP10-200kit | Hypoxyprobe-Biotin Kit (200 mg pimonidazole HCl plus 2 units of 200ul of Mouse Biotin fluorophore (HP-Biotin)) | 1 kit |
派諾硝唑探針&生物素標(biāo)記-抗派諾硝唑探針小鼠單抗 | HP10-1000kit | Hypoxyprobe-Biotin Kit (1000 mg pimonidazole HCl plus 2 units of 200ul of Mouse Biotin fluorophore (HP-Biotin)) | 1 kit |
派諾硝唑探針&PE-抗派諾硝唑探針小鼠單抗 | hp11-100kit | (100 mg pimonidazole HCl plus 2 units of mouse IgG1 monoclonal antibody conjugated to Phycoerythrin (PE) fluorophore) | 1 kit |
派諾硝唑探針&PE-抗派諾硝唑探針小鼠單抗 | hp11-200kit | (200 mg pimonidazole HCl plus 2 units of mouse IgG1 monoclonal antibody conjugated to Phycoerythrin (PE) fluorophore) | 1 kit |
派諾硝唑探針&PE-抗派諾硝唑探針小鼠單抗 | hp11-1000kit | (1000 mg pimonidazole HCl plus 2 units of mouse IgG1 monoclonal antibody conjugated to Phycoerythrin (PE) fluorophore) | 1 kit |
派諾硝唑探針&抗派諾硝唑探針價(jià)兔單抗(不帶標(biāo)記) | HP12-100kit | Kit (100 mg pimonidazole HCl plus 2 units of Rabbit IgG1 High Titer monoclonal antibody) | 1 kit |
派諾硝唑探針&抗派諾硝唑探針價(jià)兔單抗(不帶標(biāo)記) | HP12-200kit | Kit (200 mg pimonidazole HCl plus 2 units of Rabbit IgG1 High Titer monoclonal antibody) | 1 kit |
派諾硝唑探針&抗派諾硝唑探針價(jià)兔單抗(不帶標(biāo)記) | HP12-1000kit | Kit (1000 mg pimonidazole HCl plus 2 units of Rabbit IgG1 High Titer monoclonal antibody) | 1 kit |
派諾硝唑探針 | HP-100mg | 100 mg of Pimonidazole Hydrochloride solid | 100mg |
派諾硝唑探針 | HP-200mg | 200 mg of Pimonidazole Hydrochloride solid | 200mg |
派諾硝唑探針 | HP-500mg | 500 mg of Pimonidazole Hydrochloride solid | 500mg |
派諾硝唑探針 | HP-1000mg | 1000 mg of Pimonidazole Hydrochloride solid | 1000mg |
CCI-103F探針 | CCI-103F-250mg | CCI-103F (Hypoxyprobe?F6) - 0.25 gram | 250mg |
CCI-104F探針 | CCI-103F-500mg | CCI-103F (Hypoxyprobe?F6) - 0.5 gram | 500mg |
CCI-105F探針 | CCI-103F-1000mg | CCI-103F (Hypoxyprobe?F6) - 1.0 gram | 1000mg |
抗派諾硝唑探針小鼠單抗(不帶標(biāo)記) | Mouse-Mab1 | Mouse Mab1(1.0ml of mouse IgG1 monclonal antibody) | 支 |
抗派諾硝唑探針兔多抗(不帶標(biāo)記) | Pab2627 | Pab2627(200ul of Pab2627 rabbit antisera) | 支 |
FTIC-抗派諾硝唑探針小鼠單抗 | FITC-Mab | FITC Mab(200ul of conjugated IgG1 mouse monclonal antibody(clone 4.3.11.3)) | 支 |
抗CCI-103F兔多抗 | PabF6 | PabF6(200ul of diluted rabbit anti CCI-103F antisera) | 支 |
Dylight™549標(biāo)記-抗派諾硝唑探針小鼠單抗 | red-549-mab | Red 549 Mab(200ul of daylight 549per IgG1 molecule) | 支 |
APC-抗派諾硝唑探針小鼠單抗 | Mouse-APC-Mab | Mouse APC Mab(200ul of stabilized buffer at concentration mouseIgG1 monoclonal antibody of approximay 0.5mg/ml) | 支 |
生物素標(biāo)記-抗派諾硝唑探針小鼠單抗 | Mouse-Biotin-Mab | Mouse Biotin Mab(200ul of Mouse IgG1 anti-pimonidazole Biotein Mab) | 支 |
PE-抗派諾硝唑探針小鼠單抗 | redpe-mab | Hypoxyprobe™ RED PE | 支 |
抗派諾硝唑探針價(jià)兔單抗(不帶標(biāo)記) | pab27 | Hypoxyprobe PAb27 High Titer Antisera | 支 |